Coding
Part:BBa_K4275011:Design
Designed by: Zheng Xiaoyou Group: iGEM22_GreatBay_SCIE (2022-09-29)
PETase5-dockerin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 88
Illegal NgoMIV site found at 142
Illegal NgoMIV site found at 169 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
1. A 10 aa long GS linker (GGGGS)2 is appended following the Ser273 residue at the C' terminal of the original sequence.
2. The 79 amino acid long type-I dockerin domain is fused at the terminal of the GS linker, followed by a TEV site and a 6xhis affinity purification tag (HHHHHH).
Source
Ideonella sakaiensis
Clostridium thermocellum (Dockerin-I domain)
References
1. "Part:Bba K3715005 - Parts.Igem.Org". Parts.Igem.Org, 2022, https://parts.igem.org/Part:BBa_K3715005.