Coding

Part:BBa_K4275011:Design

Designed by: Zheng Xiaoyou   Group: iGEM22_GreatBay_SCIE   (2022-09-29)


PETase5-dockerin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 88
    Illegal NgoMIV site found at 142
    Illegal NgoMIV site found at 169
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. A 10 aa long GS linker (GGGGS)2 is appended following the Ser273 residue at the C' terminal of the original sequence.

2. The 79 amino acid long type-I dockerin domain is fused at the terminal of the GS linker, followed by a TEV site and a 6xhis affinity purification tag (HHHHHH).

Source

Ideonella sakaiensis
Clostridium thermocellum (Dockerin-I domain)

References

1. "Part:Bba K3715005 - Parts.Igem.Org". Parts.Igem.Org, 2022, https://parts.igem.org/Part:BBa_K3715005.